Figure 1
Reduced rejection of MHC class I− hematopoietic grafts in WASp KO NK mice.
(A) In vivo imaging of Balb/c mice injected with 1 × 106 YAC-1 lymphoma cells. Cells were labelled with the DiR dye to assess tumor growth, as a measure of fluorescence. Scale: Radiant efficiency in (p/sec/cm2/sr)/(mW/cm2). (B) YAC-1 tumor growth in Balb/c mice using in vivo imaging after 5h of injection plotted as radiant efficiency, from a pool of 3 individual experiments. Autofluorescence measured in a non-injected mouse was subtracted from injected mice at 5 hr. WT n = 8, WASp KO n = 8. (C) In vivo rejection protocol in C57BL/6 mice. MHC class I deficient splenocytes (β2m−/−) or lymphoma cells (RMA-S) were labelled with a high CFSE concentration. WT splenocytes or RMA lymphoma cells were labelled with a low CFSE concentration. A 1:1 cell mix is then injected into WT or WASp KO mice and rejection is measured after 2 days. (D) In vivo lymphoma cell rejection assay in C57BL/6 mice shown as percentage of rejected RMA-S cells, from a pool of 2 individual experiments. WT n = 5, WASp KO n = 5, β2m−/− n = 5. (E) In vivo splenocyte rejection assay in C57BL/6 mice shown as percentage of rejected β2m−/− cells, from a pool of 2 individual experiments. WT n = 10, WASp KO n = 10. Outliers based on ROUT (Q = 1%) excluded: WT n = 1.Each dot represents one mouse. Graphs show mean values ± SEM. Significance was assessed with the Mann-Whitney test. **P < 01, ***P < 001 and ns = not significant.
