Figure 3

WASp KO NK cells have increased CD69, DNAM-1, LAG-3 and KLRG1.

(A) Expression of activating and costimulatory receptors on C57Bl/6 WT and WASp KO NK1.1⁺CD3⁻ spleen NK cells. NKp46, NKG2D, NK1.1, 2B4 and DNAM-1; MFI is a pool of 3 individual experiments. WT n = 12, WASp KO n = 12. LFA-1, FAS-L and ICOS; MFI is a pool of 2 individual experiments WT = 5, WASp KO = 5. CD69; MFI is a pool of 3 individual experiments. WT n = 10, WASp KO n = 11. The gray histogram represents an unlabeled control. (B) MFI of inhibitory receptors on C57Bl/6 WT and WASp KO NK1.1⁺CD3⁻ spleen NK cells. Ly49A, Ly49C, Ly49G, Ly49I, NKG2A; MFI is a pool of 2 individual experiments is shown. WT n = 5, WASp KO n = 6. CTLA-4, PD-1 and LAG-3; MFI is a pool of 2 individual experiments. WT n = 10, WASp KO n = 10. KLRG1; MFI is a pool of 3 individual experiments. WT n = 10, WASp KO n = 11. The gray histogram represents an unlabeled control. Graphs show mean values ± SEM. Significance was assessed with the Mann-Whitney test. *P < 05, **P < 01, ***P < 001 and ns  =  not significant. Abbreviations: MFI; Mean Fluorescence Intensity.