Figure 5: IFN-γ secretion in mice following immunization with monovalent or tetravalent recombinant lipidated dengue envelope protein domain III. | Scientific Reports

Figure 5: IFN-γ secretion in mice following immunization with monovalent or tetravalent recombinant lipidated dengue envelope protein domain III.

From: Immunogenicity of a novel tetravalent vaccine formulation with four recombinant lipidated dengue envelope protein domain IIIs in mice

Figure 5

Groups of BALB/c mice (n = 3–4) were immunized subcutaneously twice with a monovalent (10 μg recombinant lipidated dengue envelope protein domain III), a tetravalent (10 μg of each serotype of recombinant lipidated dengue envelope protein domain III) vaccine, or PBS at a 4-week interval. Splenocytes were prepared one week after the last immunization. A panel of 15-mer overlapping peptides that covered the entire sequence of each dengue envelope protein domain III was used to stimulate splenocytes. The frequencies of IFN-γ-producing cells in spleens were determined by mouse INF-γ ELISPOT kits. Data represent the mean ± standard error from total 6–8 mice of 2 experiments.

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