Figure 7: Surface expression of ASIC2b is increased in the presence of ASIC2a in SH-SY5Y cells.
From: Acid-Sensing Ion Channel 2a (ASIC2a) Promotes Surface Trafficking of ASIC2b via Heteromeric Assembly
(a) Representative confocal images of SH-SY5Y cells expressing GFP-tagged ASIC2a or ASIC2b with the plasma membrane marker, RFP-PH. In SH-SY5Y cells, ASIC2a and ASIC2b are localized in the cell surface and the ER, respectively, as observed in HEK293T cells. Line scanning of fluorescent images was processed by using ZEN2011 software (Carl Zeiss). (b) Representative confocal images of SH-SY5Y cells co-expressing ASIC2a and ASIC2b. In the presence of ASIC2a, surface expression of ASIC2b was increased, as indicated by a yellow color in the merged image and line scanning. The scale bar represents 5 μm.
