Figure 3: Effect of gemcitabine in vitro and in vivo. | Scientific Reports

Figure 3: Effect of gemcitabine in vitro and in vivo.

From: Cell-cycle-controlled radiation therapy was effective for treating a murine malignant melanoma cell line in vitro and in vivo

Figure 3

Analysis of the effect of gemcitabine (GEM) on the cell cycle in vitro by flow cytometry (a). Fucci-expressing B16BL6 cells were incubated with gemcitabine and subjected to flow cytometry. Compared with the control condition, gemcitabine increased the percentage of green-fluorescing cells, and those cells had almost 2N-DNA content, indicating they were in early S phase. (b) Ratio of S/G2/M cells. Columns represent means ± SEM. Data are derived from three independent experiments. (c) Representative images of the in vivo analysis of the effect of gemcitabine on the cell cycle by two-photon microscopy. Fucci-expressing B16BL6 cells were inoculated into the abdomen of C57BL/6J mice and were treated by gemcitabine. Mice were sacrificed at the indicated time point and observed by two-photon microscopy. (d) Compared with the control, gemcitabine gradually increased the percentage of green-fluorescing cells, which was highest 12–18 h after treatment (p < 0.05). Each plot reflects one xenograft and the bars represent the average percentage. The statistical difference was analysed by Dunnett’s test.*p < 0.05, **p < 0.01.

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