Table 1 Chromosome-specific primer sets for cloning type-b ALP genes.
From: Genetic characterization of cysteine-rich type-b avenin-like protein coding genes in common wheat
Marker | Primer sequences (5′-3′) | Tm (°C) | Product size (bp) | Amplified target |
|---|---|---|---|---|
ALP | F: TGCCACACATGATGATGCATG | 60 | 912 | Full length |
R:ATGAAGGTCTTCATCCTG GCTC | ||||
ALP-7A | F: ATGCCAACATCAACAACCG | 55 | 762 | 7A genome-specific |
R: TAGTACGCACCACCAGGGTAA | ||||
ALP-4A | F: TCGGACAATACCAACAACAG | 55 | 777 | 4A genome-specific |
R: TCTAGCATGCACCACTAGTGC | ||||
ALP-7D | F: ATGAAGGTCTTCATCCTGGCT | 58 | 805 | 7D genome-specific |
R: CTAGCACGCACCACCAGT |
