Figure 5: DHED treatment does not induce proliferation of xenografts containing MCF-7Ac-1a breast cancer cells. | Scientific Reports

Figure 5: DHED treatment does not induce proliferation of xenografts containing MCF-7Ac-1a breast cancer cells.

From: Treatment with an orally bioavailable prodrug of 17β-estradiol alleviates hot flushes without hormonal effects in the periphery

Figure 5

(a) MCF-7Ac-1a breast cancer cells were injected into nude mice and left proliferate for 5 weeks. Then, 30 mice were treated with Δ4-androstenedione (Δ4A), 10 with vehicle and 10 with DHED. At the beginning of week 6, the treatment of 10 mice from 30 continued with Δ4A, the treatment in 10 mice was switched to vehicle and in 10 mice to DHED and the treatment continued for an additional 10 weeks. The tumor sizes were measured daily and at the time of euthanasia. (b) Tumors treated with Δ4A grew fast and the main tumor volume reached 1750 mm3 by the 14th weeks. Tumors originally treated with Δ4A, then switched to vehicle or DHED proliferated slower than those treated with Δ4A only and the rate of proliferation in these groups was similar to cells treated with vehicle or DHED only. There were no significant differences among vehicle-, DHED-, Δ4A-vehicle-, and Δ4A-DHED-treated groups However, the proliferation was significantly higher in the Δ4A alone group compared to all the other groups. Data represent mean ± SEM; *P < 0.05 compared with vehicle-treated OVX group.

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