Figure 2
From: Simplified three-dimensional tissue clearing and incorporation of colorimetric phenotyping
Light sheet imaging of SCM cleared hearts enables high-resolution structural visualization.
(A) A coronal section of P1 mouse heart (R26R-YFP crossed with Sln-Cre) demonstrating YFP fluorescence in the atria and part of the ventricular septum. Structural features can also be seen from tissue autofluorescence. (B) A three-dimensional rendering of the entire volume of the P1 mouse heart from A. YFP fluorescence is shown in yellow, (and tissue autofluorescence is shown in red). Trabecular networks and structural features can be visualized with light sheet microscopy. a = atrium, v = ventricle. Scale bars = 0.5 mm.
