Figure 1 | Scientific Reports

Figure 1

From: Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells

Figure 1

Expression of TINCR in BCa and expression change of TINCR after transfection of siRNAs performed by real-time qPCR.

(A) Expression of TINCR expression was examined in 49 paired human BCa tissues and corresponding noncancerous tissues. Data are represented as ΔΔCt which are the normalized real-time qPCR cycle threshold (Ct) values of TINCR between cancer tissues and pair-matched noncancerous tissues and defined as “<0” for low expression (n = 16) and “>0” for high expression (n = 33). (B) The relative expression level of TINCR was significantly higher in bladder cancer tissues compared with matched normal tissues (paired T test). Bar: mean ± SEM; *P < 0.05. (C) Compared with SV-HUC-1, the relative expression level of TINCR was overexpress significantly in BCa cell lines 5637 and SW780. Bars: mean ± SD; *P < 0.05, **P < 0.01. (D,E) TINCR expression level following the treatment of 5637 and SW780 cells with siRNAs. The si-TINCR B could significantly knock down TINCR in both 5637 and SW780. Bars: mean ± SD; *P < 0.05, **P < 0.01.

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