Figure 5 | Scientific Reports

Figure 5

From: Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells

Figure 5

Effects of the OFF device on the proliferation and apoptosis of BCa cells in vitro.

(A,B) Representative images of EdU assay in BCa cell after transfection the OFF device. (C) EdU assay manifested that the proliferation inhibition of BCa cell activated by silencing TINCR could be switched off by the OFF device at 2 mM theophylline. Bars: mean ± SD; **P < 0.01. (D,E) CCK8 assay demonstrated that growth inhibition of BCa cell activated by silencing TINCR could be turned off by the OFF device at 2 mM theophylline. P < 0.01. (F) ELISA assay supported that the activity of caspase-3 activated by silencing TINCR could be turned off by the OFF device at 2 mM theophylline. (G,H) Representative scatter plots of flow cytometry assay in BCa cell after transfection the OFF device. (I) Flow cytometry assay showed that the OFF device at 2 mM theophylline could turn off the promotion of apoptosis activated by silencing TINCR. Bars: mean ± SD; **P < 0.01.

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