Figure 3

CCR5 expression in myeloid cells in steady state condition and during colitis.

(A) CD11b⁺ cells in the colonic LP exhibit up-regulation of CCR5 during colitis which can be blocked by administration of maraviroc therapy (n = 3 per group; *P < 0.05). (B) The CD11b⁺ CCR5⁺ cell colonic infiltrate was analyzed for differential expression of MHCII and GR1; the upper panel shows a representative dot plot and the lower panel shows the relative proportions of P1, P2 and P3 cells detected in untreated and colitic mice that were treated or not with maraviroc. (C) Cytokine production by colonic LP leukocytes upon ex vivo stimulation with LPS for 36 h. Values indicate mean ± SE; n = 3/group (*P < 0.05, **P < 0.01, ***P < 0.005).