Figure 5: Time-lapse imaging of Rbfox1-iso1-deficient neuron migration. | Scientific Reports

Figure 5: Time-lapse imaging of Rbfox1-iso1-deficient neuron migration.

From: Essential role of the nuclear isoform of RBFOX1, a candidate gene for autism spectrum disorders, in the brain development

Figure 5

(a) Cortical slices at the beginning of tissue culture under the confocal microscope were shown. E14.5 cortices were coelectroporated with pCAG-EGFP with pSuper-H1.shLuc (Control) or pSuper-mRbfox1-iso1#1, followed by coronal section slice preparation at E16.5 and time-lapse imaging. There was no difference in transfection efficiency between the experiments. Scale bars in (a–d), 20 μm. (b) Time-lapse imaging of control and Rbfox1-iso1-deficient neurons at IZ-CP boundary. (c) Tracing of control or the deficient neurons (iso1#1) in upper IZ - lower CP in (b) Migratory tracks of 8 cells were demonstrated as color lines. (d) Time-lapse imaging of control and the deficient neurons (iso1#1) migrating in CP. (e) Migration distance of control and the deficient neurons (iso1#1) in CP after crossing IZ. Thirteen cells were selected in (d) and analyzed. (f) Calculation of migration velocity of control and the deficient neurons (iso1#1) in middle CP. Twenty cells were analyzed in each experiment (n = 3). Error bars indicate SD; **p < 0.01 by Student’s t-test. Analyses were repeated 3 times for each case. Representative results were shown in (a–e).

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