Figure 1
From: Genome Wide Association Study Identifies L3MBTL4 as a Novel Susceptibility Gene for Hypertension
Expression and localization of L3MBTL4.
(a) Relative mRNA expression of L3MBTL4 in Wistar-Kyoto rats (WKYs) and spontaneously hypertensive rats (SHRs) (n = 8 each group). (b) Quantification of L3MBTL4 mRNA level in the blood vessels (n = 12 per group). (c) Western blot analysis of L3MBTL4 protein expression relative to GAPDH from tissues (n = 3–6 each group). (d) Increased protein levels of L3MBTL4 in the vessel from SHR are verified by western blot (n = 4 each group). (e) Expression of L3MBTL4 mRNA in different cell lines. (f–g) Representative immunofluorescence images of L3MBTL4 (red) and α-actin (green) in the vessels from WKY and SHR (f), as well as cultured vascular smooth muscle cells (g). Nuclei are stained with DAPI (blue). Scale bars are 50 μm. n = 3 independent experiments for (e–g), *p < 0.05, **p < 0.01, ***p < 0.001 compared to WKY. All data represent mean ± s.e.m.
