Figure 6: NM1 links the plasma membrane to underlying cytoskeletal structures.

(A) 3D cell reconstructions of representative cells with different morphological features are shown. Colours represent the relative height values in each of the respective images (black – lowest, white – highest). (B) Height maps of representative NM1 KO and WT cells with highlighted membrane regions that were included in the analysis are shown. (C) Density plots of YM values calculated from highlighted areas of the cell surface are shown. They are in agreement with previous results, showing a wide distribution of YM values in WT in comparison to the narrow range of YM in KO cells. (D) Elasticity maps show striking differences in spatial distributions of YM values measured in NM1 KO and WT cells. The position and shape of actin filaments is reflected in elasticity maps of WT membranes, while in KO cells no such influence from the actin cytoskeleton can be seen. The colour scale shows increasing YM values.