Figure 1: Nanosecond Pulsed Laser Experiments.
From: Excitation Light Dose Engineering to Reduce Photo-bleaching and Photo-toxicity
Case 1: Equal experiment duration with continuous or pulsed laser excitation of equal peak power. Case 2: Equal total light exposure was created by exposing the sample longer if the light source was pulsed but keeping the peak light power constant. The experimental time was thus longer for pulsed light experiments but the total time the sample was exposed to light was constant. Case 3: Equal total light exposure was created by exposing the sample with single line scans and a long pixel dwell time or multiple line scans with a short pixel dwell time. The peak power and the total light exposure at each pixel location were kept constant.
