Figure 4: Rapid line scanning reduces photo-bleaching.
From: Excitation Light Dose Engineering to Reduce Photo-bleaching and Photo-toxicity
(A) CHO-K1 cells expressing paxillin-EGFP were fixed with PFA and imaged with 2% power from the 488 nm laser on the CLSM with variable numbers of line scans. (B) Using 5% laser power and keeping the pixel dwell time constant but scanning rapidly 8 times or slowly 1 time. (C) Photo-bleaching curves for one slow scan or 2, 4, 8 or 16-line scans. (D) Average normalized decay rates in images−1 for various scan speeds and numbers. Scale bars are 10 μm in A and 2 μm in B. Error bars in (D) are standard error of the decay fitting. P values were calculated using a t-test of means and are shown relative to the 1-scan setting. *represents P < 0.05 and ***P < 0.001.
