Figure 5: Rapid line scanning and mitochondrial morphology and membrane potential.
From: Excitation Light Dose Engineering to Reduce Photo-bleaching and Photo-toxicity
(A) Live CHO-K1 cells expressing paxillin-EGFP were stained with MitoTracker RedTM. The mitochondrial morphology was imaged with the 543 nm laser at 1% power before and after 100 consecutive image scans of paxillin-EGFP were collected with the 488 nm laser line at 20% power. (B) TMRM controls with DMSO loading control, FCCP to depolarize the mitochondrial membrane or oligomycin to hyper-polarize the mitochondrial membrane. (C) Percentage decrease in 3D TMRM intensity following exposure of each cell for 100 images with the 488 nm laser line at 20% power. Scale bars are 10 μm in A and 50 μm in B. Error bars in (C) are SEM. One *represents P < 0.05.
