Figure 2
From: Fusion to a homo-oligomeric scaffold allows cryo-EM analysis of a small protein
Negative-stain EM analysis of MBP-GS fusion proteins.
(A) Electron micrographs of fusion proteins stained with sodium silicotungstate. Top and side views of particles are circled in magenta and green, respectively. Scale bar, 50 nm. (B) Magnified view of particles circled in (A). The two most promising constructs identified by biophysical analysis are boxed. (C) Class averages for top and side views of constructs Δ2 and Δ5 obtained by analysis of a small dataset (400 particles per construct). For comparison, the predicted projection of isolated GS is shown in the same orientation.
