Figure 3
From: Fusion to a homo-oligomeric scaffold allows cryo-EM analysis of a small protein
Initial cryo-EM analysis of MBP-GS constructs Δ2 and Δ5.
(A,B) Representative class averages showing top and side views for MBP-GS constructs (A) Δ2 and (B) Δ5. (C,D) Fit of the MBP and GS crystal structures into the cryo-EM reconstructions obtained for construct (C) Δ2 and (D) Δ5. Both maps are countoured at 1.5 σ. The N- and C-terminal domains of MBP are labelled NTD and CTD and colored in purple and magenta, respectively. The GS subunit is in cyan. For construct Δ5, the central plug of density and the MBP region that lies outside the map are indicated in red.
