Figure 4
From: Fusion to a homo-oligomeric scaffold allows cryo-EM analysis of a small protein
Final cryo-EM reconstruction of MBP-GS construct Δ2.
(A) Representative class averages showing top and side views. (B) FSC curve of the 3D reconstruction (calculated prior to masking). (C) Local resolution plotted onto the 3D reconstruction colored from blue (≤3 Å) to red (≥10 Å). Insets show different cross-sections of the MBP and GS subunit rings (boxed in black) or of an MBP-GS monomer (boxed in magenta). (D) Fit of the MBP and GS crystal structures into the 3D reconstruction. The N- and C-terminal domains of MBP (NTD, CTD) are in purple and magenta, respectively; the GS subunit is in cyan. Insets show the cryo-EM map covering one MBP or GS subunit. The 3D reconstruction is displayed at two different isosurface levels for each domain. Contour levels are 2.5 σ (gray), 4.2 σ (cyan) and 6.5 σ (green). (E) Stereo view showing the active site of MBP, with maltose indicated in stick representation. The map is contoured at 4.6 σ.
