Figure 1 | Scientific Reports

Figure 1

From: Hypoxic Preconditioning Inhibits Hypoxia-induced Apoptosis of Cardiac Progenitor Cells via the PI3K/Akt-DNMT1-p53 Pathway

Figure 1

Characterization of cultured CPCs.

(a) Cells (small, round, phase-bright) migrated from the cardiac explants, aggregated and proliferated over the fibroblast layer after 10 days of culture. Scale bar: 50 μm. (b) Phase-contrast image of CPCs after magnetic-activated cell sorting for 1 day. (c) Phase-contrast image of CPCs after magnetic-activated cell sorting for 3 days. (d) Representative clone generated by CPCs after magnetic-activated cell sorting for 7 days. (e) Representative flow cytometric analyses of CPCs for positive expression of the cell surface markers c-kit and Sca-1. (f) Representative flow cytometric analyses of CPCs for negative expression of the cell surface markers CD34 and CD45.

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