Figure 2: Depletion of CIB proteins impairs HIV-1 replication in Jurkat T-cells.
From: CIB1 and CIB2 are HIV-1 helper factors involved in viral entry
Each shRNA population was infected with 2.5 ng of HIV-1NL4-3. On days 5 and 7 after infection, cells were stained with anti-Gag-PE antibody and analyzed by flow cytometry to quantify the percentage of intracellular Gag (iGag) positive cells (A). Viral replication was also assessed by quantifying HIV Gag levels in supernatant by p24 ELISA on days 5 and 7 of infection (C). The corresponding area under the curve (AUC) from replication kinetics is also shown (B,D). Values represent mean ± SEM of 4 or more independent transductions and are expressed relative to values obtained for non-transduced (NT) cells. *P < 0.05, **P < 0.01 versus sh-SCRAM (Mann-Whitney test).
