Figure 3: Impairment of HIV replication is specific to downmodulation of CIB proteins.
From: CIB1 and CIB2 are HIV-1 helper factors involved in viral entry
To restore CIB protein levels in cells expressing shRNAs targeting CIB proteins, cells were co-transduced with viral vectors leading to the expression of shRNAs and viral vectors carrying RNAi-resistant cDNA for CIB1 or CIB2. Total RNA was extracted and cDNA was synthesized for quantification by quantitative real-time PCR. mRNA levels for CIB1 (A) and CIB2 (B) are relative to levels present in non-transduced (NT) Jurkat cells. Each co-transduced cell population was infected with 2.5 ng of HIV-1NL4-3, and viral replication was assessed by measuring the percentage of intracellular Gag (iGag) positive cells by flow cytometry on days 5 and 7 after infection. Results for CIB1-complemented cells (C) and CIB2-complemented cells (D) are shown. Values represent mean ± SEM of 3 or more independent transductions.
