Figure 2 | Scientific Reports

Figure 2

From: Efficient Suppression of Hepatitis C Virus Replication by Combination Treatment with miR-122 Antagonism and Direct-acting Antivirals in Cell Culture Systems

Figure 2

In vitro selection experiments in genotype 1a HCV-replicating cells.

Huh-7.5 cells harboring tat/2A-Neo-H77S were treated with mock, DAA (simeprevir, daclatasvir or sofosbuvir), LNA–anti-miR-122, or DAA plus LNA–anti-miR-122 in the presence of G 418. The concentration of DAA was gradually increased and LNA–anti-miR-122 was transfected into Huh-7.5 cells twice a week at cell passage. Schematic representations of the experiment using tat/2A-Neo-H77S-replicating cells with simeprevir (a), tat/2A-Neo-H77S-replicating cells with daclatasvir (c) and tat/2A-Neo-H77S-replicating cells with sofosbuvir (e). The amount of HCV RNA was normalized to that of β-actin mRNA in each group (b,d,f). The arrows in the upper of each figure represent the time points of LNA-anti-miR-122 transfection and DAA addition. Results are shown as means + SEM.

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