Figure 3 | Scientific Reports

Figure 3

From: microRNA-132/212 deficiency enhances Aβ production and senile plaque deposition in Alzheimer’s disease triple transgenic mice

Figure 3

Regulation of Aβ by miR-132 and its target Sirt1.

(a,b) ELISA of human Aβ40 and Aβ42 in Neuro2a-APPSwe and HEK293-APPSwe cells after miR-132 treatment (n = 2 cultures in triplicate per group). (c) Western blot analysis of Sirt1 in Neuro2a-APPSwe and HEK293-APPSwe cells following miR-132 transfection (n = 2 cultures in triplicate per group). Total proteins were used as normalization control. (d,e) ELISA of human Aβ40 and Aβ42 in HEK293-APPSwe cells after Sirt1 pharmacological inhibition (EX527) or genetic downregulation (si-Sirt1) (n = 2 cultures in triplicate per group). Data are presented as mean ± SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Student’s t-test). Full-length blots/gels are presented in Supplementary Fig. S6.

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