Figure 3
From: Mitofusin-2 is required for mouse oocyte meiotic maturation
Mfn2 siRNA causes defects of the meiotic spindle and chromosome alignment.
(A) Oocytes microinjected with Mfn2 or control siRNA were incubated in M2 medium containing 2.5 μM milrinone for 24 h and then transferred to milrinone-free M2 for 12 h. This was followed by immunostaining with an α-tubulin antibody (green) and Hoechst 33342 (red). (B,C) The percentages of oocytes with abnormal spindles or misaligned chromosomes in siRNA-injected and control oocytes. Data are expressed as means ± SEM of at least three independent experiments. *Significantly different from control (p < 0.05).
