Figure 3
From: Addressing proteolytic efficiency in enzymatic degradation therapy for celiac disease
Nepenthes fluid proteases accelerate the clarification rate of gliadin slurries.
(a–c) Time-course digestions of 10 mg/ml gliadin (pH 2.5 at 37 °C) with the indicated micromolar concentrations of (a) pepsin, (b) Nepenthes fluid proteases, or (c) Nepenthes fluid proteases supplemented with 5 μM pepsin. Turbidity of the digested gliadin was monitored as absorbance at 595 nm (A595). Black lines in (a–c) represent the turbidity of the gliadin slurry in the absence of protease. Measurement precision was <3% RSD (n = 3). (d) Slurry clarification rates, measured at 90% of the maximal effect, for digestions using Nepenthes fluid enzymes alone and pepsin alone. Data taken from (a,b). Values reported as milligrams of gliadin per micromolar enzyme concentration per minute digestion time.
