Figure 5: T3 antagonizes IL-6 signaling in Hep3B cells.
From: The Thyroid Hormone Receptors Inhibit Hepatic Interleukin-6 Signaling During Endotoxemia
(A) Proteins were extracted from Hep3B cells pretreated with 5 nM T3 in 10% thyroid hormone-depleted serum for 36 h and then treated with 10 ng/ml IL-6 for times ranging from 0 to 120 min. The levels of phosphorylated and total STAT3 and ERK were assessed by Western blot. (B) Western blot analysis of the indicated proteins in cells pretreated with T3 and then incubated with 10 ng/ml TNFα for varying times. Full-length blots are presented in Supplementary Fig. 9. (C) Cells were transiently transfected with a reporter plasmid containing STAT binding sites and incubated in the presence and absence of T3 for 36 h and with IL-6 for the last 5 h. (D) Luciferase activity in cells transfected with a reporter plasmid containing NF-kB binding sites and treated with T3 for 36 h and/or TNFα for the last 5 h.
