Figure 4
From: In Vivo Imaging of Retinal Hypoxia in a Model of Oxygen-Induced Retinopathy
Effect of HYPOX-4 on retinal physiology was assayed in RA-raised mice via electroretinography (ERG) analysis.
(A,B) ERG measurements of dark-adapted mice 7 days post systemic administration of HYPOX-4 revealed no significant changes in mean a-wave and b-wave amplitudes at various flash intensities compared to vehicle (PBS) and sodium fluorescein (control) groups. The TUNEL assay was performed in retinal transverse sections to assess retinal apoptosis and taken as a measure of retinal toxicity. RA mice were treated with 100 μM HYPOX-4 or DNase 1; HYPOX-4 showed no cellular apoptosis. (C,F) DAPI staining of nuclei; (D) DNase 1-treated retinal transverse sections serving as a positive control, fragmented DNA was clearly visible; (G) HYPOX-4 treated retinal transverse sections showed no cellular apoptosis; (E) C and D merged; (H) F and G merged. (I,J) In vitro cellular proliferation was assessed by the BrdU assay using HYPOX-4 treated R28 and MIO-M1 cells. No effect on cellular proliferation was observed.
