Figure 2 | Scientific Reports

Figure 2

From: Annexin A4-nuclear factor-κB feedback circuit regulates cell malignant behavior and tumor growth in gallbladder cancer

Figure 2

ANXA4 knockdown affected gallbladder cancer cell growth and apoptosis in vitro.

Knockdown of endogenous ANXA4 expression was confirmed by RT-PCR (A) and western blot (B). GAPDH as an internal control. Expression levels of ANXA4 mRNA and protein were obviously down-regulated in ANXA4-knockdown clones (shA4 clone number). Scr., scrambled control, expressing non-targeting control shRNA. Non-transfected cells as control. The effects of shRNA-mediated knockdown of ANXA4 in GBC-SD and NOZ cells on malignant behavior were examined using several methods. Data are presented as mean ± SD compared with the control. (C) Cell proliferation was assessed by methylthiazol tetrazolium assay. ANXA4-knockdown cells proliferated at significantly more slowly than non-transfected cells (**P < 0.01). (D) Colony formation assays. Colony formation in GBC-SD and NOZ cells was significantly inhibited after transfection with ANXA4 shRNA (**P < 0.01). (E) Cell apoptosis assessed by flow cytometry. ANXA4 shRNA increased apoptotic rates of GBC-SD and NOZ cells (**P < 0.01).

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