Figure 3

Effect of ANXA4 knockdown on caspase activation in GBC-SD and NOZ cells.
Caspase-3 and -9 activities were determined by caspase activity assay in GBC-SD (A) and NOZ (B) cells. Caspase-3 and -9 activities were significantly increased in ANXA4-knockdown cells. Data are presented as mean ± SD from three independent experiments. **P < 0.01 compared with control. (C) Pprotein expression levels of total caspase-3 and -9 were decreased in ANXA4-knockdown clones, while their cleaved forms increased, as shown by western blot analysis.