Figure 4
From: p18 inhibits reprogramming through inactivation of Cdk4/6
Knockdown of Cdk4 and Cdk6 blocks reprogramming.
(A) Transfection with a Cdk4-specific shRNA significantly inhibits Cdk4 mRNA expression. Data are normalized to cells transfected with scramble shRNA and shown as mean ± SD; n = 3, ***P < 0.001. (B) Transfection with a Cdk6-specific shRNA significantly inhibits Cdk6 mRNA expression. Data are normalized to cells transfected with scramble shRNA and shown as mean ± SD; n = 3, ***P < 0.001. (C) AP staining of iPSC colonies generated from control cells (scramble), Cdk4 knockdown cells or Cdk6 knockdown cells. (D) Statistical analysis of the effects of Cdk4/6 inhibition on iPSC colony formation. Data are normalized to control cells and shown as mean ± SD; n = 3, **P < 0.01.
