Figure 5
From: p18 inhibits reprogramming through inactivation of Cdk4/6
p18 inhibits reprogramming through Cdk4/6.
(A) Sequences of wild-type (WT) and mutant (D68N) p18 proteins. (B) Western blot analysis of whole cell extracts from MEFs transfected with vector, WT p18 or D68N mutant p18. Actin serves as a loading control. (C) AP staining of iPSC colonies generated by overexpression of WT or D68N mutant p18. Transfection with empty vector serves as a control. (D) Statistical analysis of the effect of WT p18 or p18D68N mutant expression on iPSC colony formation. Data are normalized to control cells (vector) and shown as mean ± SD; n = 3, *P < 0.05, **P < 0.01.
