Figure 6
Electron microscopy reveals OA-LUV mediated misfolding of IAPP yields IAPP fibrils in some cases decorated with OA-LUVs.
12.5 μM IAPP was allowed to misfold in the presence of 500 μM OA-LUVs. Samples were incubated for the amount of time required to achieve ThT positivity, as in Fig. 1, before being applied to the micrograph grid. (a–d) EM micrographs of IAPP in 10 mM phosphate buffer, pH 7.4 with 66 (a), 25 (b), 10 (c) and 1 mol% (d) OA-LUVs show fibrils decorated with LUVs, in some cases the shape of the LUVs are distorted where they contact fibrils. Lipid free sections of fibrils measure diameters of ~7 nm. In many cases lateral assemblies of fibrils are evident [as are small spherical structures 5 nm in diameter (arrows)]. Scale bars = 200 nm.
