Figure 7
Monobenzyl ester phthalate-LUVs enhance IAPP misfolding kinetics.
ThT was used to follow IAPP aggregation kinetics. (a) IAPP aggregation was measured as a function of time and demonstrates dependence upon the mol% of MBzP. (a) Representative ThT curves from experiments with 12.5 μM human IAPP and 500 μM MBzP-LUVs in 10 mM phosphate buffer pH 7.4 demonstrate acceleration of fibrilization for 66 (omitted in A), 25 (□), 10 (Δ), 1 mol% MBzP (x) and 100% POPC (○). (b) Comparison of t50 values from experiments in A. Error bars represent one standard deviation from a minimum of 3 experiments per condition. (p < 0.01, for all conditions compared with 100% POPC LUV control).
