Figure 2

PD0956 is a serine protease expressed in Xylella fastidiosa.
(a) Immunoblot detection of secreted PrtA. A cropped blot is shown in this figure and the whole blot image is shown in the Supplementary Information file. (b) Fluorescent protease activity assay (FTC-Casein) showing reduced activity in secreted extracts of X. fastidiosa prtA mutant vs wild type Temecula1. Average values and standard deviations of three independent experiments are plotted (*p-value < 0.05, two-tailed T-test).