Figure 7
PrtA is an antivirulence factor in Pierce’s disease.
(a) Plants infected with wild type Temecula1 and prtA mutant strain at eight weeks post-inoculation and (b) detached leaves harvested eight nodes above point of inoculation. (c) Quantification of symptoms averaged from 25 plants/strain infected with X fastidiosa prtA or wild type Tem1 control. Data is shown as the difference from uninfected control. Plotted values are averages and standard deviations from all plants tested (*p-value < 0.05, two-tailed T-test). (d) Comparison of leaf scorching symptoms in X. fastidiosa-infected wild type SR1 tobacco and SR1 expressing recombinant PrtA, after 11 weeks infection. (e) Quantification of leaf scorching area in wild type and PrtA-expressing SR1 tobacco. Plotted values are averages and standard deviations from all symptomatic leaves (n = 10 for WT SR1 tobacco and n = 10 for PrtA-expressing tobacco plants) from six plants of each genotype tested (*p-value < 0.05, two-tailed T-test). (f) Accumulated biomass of new vine buds grown for four weeks and then pruned. Plotted values are averages and standard deviations from total new biomass from a random subset (n = 12) of plants (*p-value < 0.05, ANOVA).
