Figure 1 | Scientific Reports

Figure 1

From: Characterization of new RNA polymerase III and RNA polymerase II transcriptional promoters in the Bovine Leukemia Virus genome

Figure 1

In vivo binding of RNAPIII to the BLV miRNA cluster.

(a) Schematic representation of the BLV provirus in latently-infected L267 ovine cells. The localization of the different amplified regions is presented with respect to the BLV genome. (b–g) Chromatin prepared from L267 cells (b,d–g), BLV-infected ovine PBMCs (c) or L267LTaxSN cells (f) was immunoprecipitated with specific antibodies directed against different subunits of the RNAPIII as indicated, the largest subunit of the RNAPII or with an IgG as background measurement. Purified DNA was then amplified with oligonucleotide primers hybridizing to either the BLV genome, its host cellular surrounding DNA environment (b–d,f) or to the two class III genes encoding 5S rDNA (e) and tRNAAsp (g). Results are presented as histograms indicating percentages of immunoprecipitated DNA compared to the input DNA (% IP/INP; b–e,g) or indicating fold enrichment above the value obtained with IgG, which was arbitrarily assigned the value of 1 (f). Data are the means ± SEM from one representative of at least three independent experiments.

Back to article page