Figure 5 | Scientific Reports

Figure 5

From: Characterization of new RNA polymerase III and RNA polymerase II transcriptional promoters in the Bovine Leukemia Virus genome

Figure 5

Evaluation of the CpG dinucleotide methylation status of the BLV miRNA cluster.

Genomic DNA from L267 cells was extracted and treated with sodium bisulfite and the miRNA cluster was amplified by nested PCR. These amplified products were cloned in a TA cloning vector and 10 independent clones were sequenced. Open and filled circles represent non-methylated and methylated CpG dinucleotides, respectively. The position of the 5 BLV-pre-miRs with respect to the miRNA cluster is presented in the lower part of the figure.

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