Figure 6 | Scientific Reports

Figure 6

From: Characterization of new RNA polymerase III and RNA polymerase II transcriptional promoters in the Bovine Leukemia Virus genome

Figure 6

The BLV miRNA cluster and 3′LTR/host genome junction are associated with active epigenetic marks.

Chromatin prepared from L267 cells was immunoprecipitated with specific antibodies directed against different histone post-translational modifications (a,c), the H2A.Z histone variant (b) or with an IgG as background measurement. Purified DNA was then amplified with oligonucleotide primers hybridizing to the 5′LTR, the miRNA cluster or the 3′LTR/host junction regions (a,c) or to the promoter regions of the ZNF12 and HOXD10 genes (b). Results are presented as histograms indicating percentages of immunoprecipitated DNA compared to the input DNA (% IP/INP). Data are the means ± SEM from one representative of at least three independent experiments.

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