Figure 7

The RNAPII promoter in the 3′LTR is transcriptionally active.

Raji cells were transiently co-transfected with (a) 400 ng of pREP-luc, pREP-LTR(wt)-S-luc or pREP-LTR(wt)-AS-luc constructs, increasing amounts of an expression vector for Tax (50 or 150 ng of plasmid DNA) and 20 ng of pRL-TK or with (b) 400 ng of pREP-luc, pREP-LTR(wt) -AS-luc or pREP-LTR_(MTE/DPE*)-AS-luc, pREP-LTR_(BRE*)-AS-luc or pREP-LTR_{(MTE/DPE* & BRE*)}-AS-luc mutated constructs and 20 ng of pRL-TK. Forty-eight hours after transfection, cells were collected, lysed and both firefly and renilla luciferase activities were measured. Results are presented as histograms indicating relative luciferase activities compared to the pREP-luc for wich a value of 1 was assigned. Data are the mean ± SEM from one representative of at least three independent experiments.