Figure 7
From: Control of cortex development by ULK4, a rare risk gene for mental disorders including schizophrenia
Ulk4R cDNA successfully rescues the defective radial migration mediated by shRNA268.
(A) Validation of Ulk4R cDNA. Panels of plasmids containing different DNA constructs were co-transfected into HEK293 cells. Western blotting with anti-c-Myc (top row) and anti-flag (mid-row) indicated that Ulk4R cDNA successfully restores the expression of Ulk4. Lane 1: co-transfection with plasmids containing control shRNA and Ulk4 cDNA; Lane 2: co-transfection with Ulk4 cDNA and shRNA268; Lane 3: co-transfection with control shRNA and Ulk4R cDNA; Lane 4: co-transfection with plasmids containing shRNA268 and Ulk4R cDNA. (B–D) Compared with the control group (B), the defective radial migration caused by shRNA268 (C) can be successfully rescued by Ulk4R cDNA (D). Bars = 100 μm in (B–D).
