Figure 2

Treatment with ICT-2552 results in a significant initial temperature drop, whilst use of either therapy leads to a significant drop in tumour temperature relative to the tumour volume over the longer time period.

(a) Evaluation of the efficacy of ICT-2552 and doxorubicin in terms of relative tumour volume over time (volume as a multiplicative factor of volume at day 0) based on calliper measurements. Results show a significant reduction in tumour volume for the 2 treatments relative to the control. (b) Plot of temperature versus time for all three treatment groups over the first 6 hours; curves show the mean of the tumour temperatures within the group. There is a temperature reduction for all three groups, but this is significantly greater for ICT-2552 than doxorubicin (p = 0.006) and the controls (p < 0.001). (c) plot of time versus temperature for daily time points; curve shows mean temperature for tumours within each group. A recovery is seen after 24 hours and then a gradual decrease in temperature as the tumour grows across all groups (p < 0.01 for all groups). (d) Scatter plot of temperature versus tumour volume for the three treatment groups, with lines of best fit shown. While tumour size is proportional to tumour temperature for all groups, we found that the slope of the line-of-best-fit was much steeper for the treated groups (p < 0.001).