Figure 1
From: Rapid flow cytometric measurement of protein inclusions and nuclear trafficking
FloIT detects inclusions formed by a wide variety of proteins.
(a) Two parameter, pseudo-colour flow cytometry plots showing identification of nuclei and non-nuclear particles (indicated) using FSC and RedDot2 fluorescence (left: unstained, right: stained with RedDot2). (b) Non-nuclear particles (gated as shown in (a)) analysed for eGFP fluorescence versus FSC in lysates prepared from cells transfected to express eGFP (left) or SOD1G93A-eGFP (right). SOD1G93A-eGFP inclusions (indicated) are identified by their increased eGFP fluorescence. (c) Detection of inclusions (FloIT, left) and cells containing inclusions (PulSA, right) formed by various proteins. Data are means (n = 3) ± SEM (too small to be visible in some cases). All experiments used N2a cells and each result is representative of two or more independent experiments.
