Figure 3
From: Rapid flow cytometric measurement of protein inclusions and nuclear trafficking
FSC calibration microspheres can be used to estimate the sizes of protein inclusions in FloIT analyses.
(a) FSC histograms of each of the beads (diameters indicated in key). (b) Over the size range tested, FSC and bead size displays a linear relationship (r2 = 0.99). (c) Pseudocolour plots of analyses of mCherry fluorescence versus FSC for lysates prepared from N2a cells transfected to express mCherry (which does not aggregate to form inclusions, left) or Htt25Q-mCherry (which forms limited numbers of inclusions, middle) or Htt46Q-mCherry (which forms large numbers of inclusions, right). A range of sizes of inclusions can be resolved, however the absolute sizes of those with diameters less than ~ 0.5 μm cannot be determined using standard flow cytometers (see text). Results shown are representative of three independent experiments.
