Figure 4

FloIT and manual counting provide similar estimates of the number of inclusions in cells.

(a) The number of inclusions in N2a cells expressing Htt^46Q–mCherry or FLUC^WT–eGFP was enumerated by FloIT or manual counting. Values are means + SEM (n = 3). Differences were not significant, analysed by Student’s t-test. (b) Quantification of inclusions in cells expressing TDP-43^M337V–tGFP and treated with 10 μM MG132, by FloIT (100,000 events acquired) versus manual counting (3 × 100 cell images). Values plotted are means ± SEM (n = 3). Each result shown is representative of two independent experiments.