Figure 6
From: Rapid flow cytometric measurement of protein inclusions and nuclear trafficking
FloIT can quantify nuclear flux of fluorescently tagged proteins.
(a) Time-dependent efflux of TDP-43M337V-tGFP from the nuclei of MG132 treated N2a cells, shown as % change in tGFP-fluorescing nuclei (at t = 0, the % value represents the transfection efficiency). (b) Dose-dependent influx of NFAT-eGFP into 1 µM ionomycin-treated HEK293 cell nuclei, shown as % NFAT-eGFP-positive nuclei. (c) Confocal microscopy images of NFAT-eGFP-expressing HEK293 cells with and without 1 µM ionomycin treatment. Scale bar is 10 μm. Values are means (n = 3) ± SEM; each result is representative of two or more independent experiments.
