Figure 1 | Scientific Reports

Figure 1

From: A CRISPR-Cas9 sex-ratio distortion system for genetic control

Figure 1

Generation of a CRISPR-Cas9 sex-ratio distortion system.

(a) CRISPRSD transformation construct. (pBac) piggyBac inverted repeats; (3xP3::DsRed) Pax promoter driving the DsRed marker to select gene integration events; (β2::Cas9) Streptococcus pyogenes Cas9 nuclease under the control of the male germline specific β2 tubulin promoter; (U6::gRNA) gRNA under the control of the ubiquitous U6 Pol III promoter. (b) Schematic representation of rDNA clusters within the Anopheles gambiae X-chromosome and the location of the multicopy gRNA target site. By shredding the X-chromosome during meiosis it is predominantly Y-bearing sperm that fertilize eggs generating a male-biased progeny.

Back to article page