Figure 2
Antibacterial effects of Bmattacin2.
(a) Antimicrobial activities (MICs) of Bmattacin2 against a panel of Gram-positive and Gram-negative bacteria (E. coli DH5α, E. coli ATCC25922, S. maicesceis), including three Gram-negative bacteria and two Gram-positive bacteria (S. aureus ATCC25923 and B. bombysepticus). (b) Bmattacin2-mediated growth inhibition of bacteria was conducted by recording the OD value at 600 nm after incubation of 18 hours. The growth rates among the different bacteria strains in a range of concentration of Bmattacin2 were normalized by control group (b). Data was expressed as mean with standard deviation of at least three replicates. (c) Live/dead staining of E. coli ATCC25922 and S. aureus ATCC25923 (green refer to live cells, red refer to dead cells) after treated with Bmattacin2 at their MIC for 18 hours respectively. Bar = 50 μm. (d) The effects of Bmattacin2 against surface of E. coli ATCC25922 and S. aureus ATCC25923 were evaluated through SEM observation. Bar = 1 μm.
