Figure 10 | Scientific Reports

Figure 10

From: Dimethyl fumarate blocks pro-inflammatory cytokine production via inhibition of TLR induced M1 and K63 ubiquitin chain formation

Figure 10

Effect of DMF on IL-1 and TNF stimulated M1 chain formation.

(A) IL-1R HEK293 cells were incubated with 50 μM DMF for 4 h where indicated. Cells were stimulated with 5 ng/ml IL-1β for 30 min and the levels of phospho p105, phospho IKK, total IκBα, phospho ERK1/2 and total ERK1/2 determined by immunoblotting. (B) As (A) but Halo-NEMO beads were used to pull down M1 polyubiquitin chains. Pulldowns were blotted for M1 and K63 linked polyubiquitin chains. (C) HeLa cells were incubated with 50 μM DMF for 4 h where indicated. Cells were stimulated with 10 ng/ml TNF for 30 min and the levels of phospho p105, phospho IKK, total IκBα, phospho ERK1/2 and total ERK1/2 determined by immunoblotting. (D) As (C) but Halo-NEMO beads were used to pull down M1 polyubiquitin chains. Pull downs were blotted for M1 and K63 linked polyubiquitin chains.

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